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kank3_pJet1.2
(Plasmid #121981)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 121981 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pJet1.2
  • Vector type
    Cloning Vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    kank3
  • Alt name
    si:dkeyp-104h9.6
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    2742
  • Entrez Gene
    kank3 (a.k.a. CH211-243P7.1, si:dkeyp-104h9.6)
  • Promoter T7

Cloning Information

  • Cloning method TOPO Cloning
  • 5′ sequencing primer pJET1.2 forward
  • 3′ sequencing primer pJet1.2 Reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid contains a nonstop mutation compared to the Kank3 protein coding sequence but this does not impact its use as a riboprobe DNA template.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    kank3_pJet1.2 was a gift from GuangJun Zhang (Addgene plasmid # 121981 ; http://n2t.net/addgene:121981 ; RRID:Addgene_121981)

  • For your References section:

    Evolutionary and developmental analysis reveals KANK genes were co-opted for vertebrate vascular development. Hensley MR, Cui Z, Chua RF, Simpson S, Shammas NL, Yang JY, Leung YF, Zhang G. Sci Rep. 2016 Jun 13;6:27816. doi: 10.1038/srep27816. 10.1038/srep27816 PubMed 27292017
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