kank1a_pJet1.2
(Plasmid
#121978)
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Purposefor generating kank1a riboprobes for WISH in zebrafish
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 121978 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepJet1.2
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Vector typeCloning Vector
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namekank1a
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Alt namewu:fc93h12
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Alt namezgc:153166 or si:dkey-146l2.1
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SpeciesD. rerio (zebrafish)
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Insert Size (bp)3900
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Entrez Genesi:dkey-146l2.1 (a.k.a. zgc:153166)
- Promoter T7
Cloning Information
- Cloning method TOPO Cloning
- 5′ sequencing primer pJET1.2 forward
- 3′ sequencing primer pJet1.2 Reverse (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
kank1a_pJet1.2 was a gift from GuangJun Zhang (Addgene plasmid # 121978 ; http://n2t.net/addgene:121978 ; RRID:Addgene_121978) -
For your References section:
Evolutionary and developmental analysis reveals KANK genes were co-opted for vertebrate vascular development. Hensley MR, Cui Z, Chua RF, Simpson S, Shammas NL, Yang JY, Leung YF, Zhang G. Sci Rep. 2016 Jun 13;6:27816. doi: 10.1038/srep27816. 10.1038/srep27816 PubMed 27292017