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mdm1_pJet1.2
(Plasmid #121977)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 121977 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pJet1.2
  • Vector type
    Cloning Vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mdm1
  • Alt name
    arrd2
  • Alt name
    Mdm-1
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    1971
  • Entrez Gene
    mdm1 (a.k.a. si:ch211-266a5.6)
  • Promoter T7

Cloning Information

  • Cloning method TOPO Cloning
  • 5′ sequencing primer pJET1.2 forward
  • 3′ sequencing primer pJet1.2 Reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mdm1_pJet1.2 was a gift from GuangJun Zhang (Addgene plasmid # 121977 ; http://n2t.net/addgene:121977 ; RRID:Addgene_121977)

  • For your References section:

    Molecular Evolution of MDM1, a "Duplication-Resistant" Gene in Vertebrates. Hensley MR, Chua RF, Leung YF, Yang JY, Zhang G. PLoS One. 2016 Sep 22;11(9):e0163229. doi: 10.1371/journal.pone.0163229. eCollection 2016. PONE-D-15-54181 [pii] PubMed 27658201
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