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Addgene

Type I inositol-1,4,5-trisphosphate 5-phosphatase in pcDNA3
(Plasmid #121976)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 121976 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Type I Inositol-1,4,5-trisphoosphate 5-phosphatase
  • Alt name
    INPP5A
  • Species
    H. sapiens (human)
  • GenBank ID
    X77567.1
  • Entrez Gene
    INPP5A (a.k.a. 5PTASE)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site EcoRV (unknown if destroyed)
  • 5′ sequencing primer TAATACGACTCAC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Type I inositol-1,4,5-trisphosphate 5-phosphatase in pcDNA3 was a gift from Christophe Erneux (Addgene plasmid # 121976 ; http://n2t.net/addgene:121976 ; RRID:Addgene_121976)

  • For your References section:

    Post-translational modification of human brain type I inositol-1,4,5-trisphosphate 5-phosphatase by farnesylation. De Smedt F, Boom A, Pesesse X, Schiffmann SN, Erneux C. J Biol Chem. 1996 Apr 26;271(17):10419-24. PubMed 8626616
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