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Addgene

GBSGFP in pBS-KS
(Plasmid #121974)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 121974 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBS-KS
  • Modifications to backbone
    8xdel-hsp68-EGFP-pA
  • Vector type
    cloning

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GBSGFP
  • Alt name
    GBS(long)-GFP
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Gnb5 (a.k.a. GBS, Gbeta5, Hg2e, flr)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GBSGFP in pBS-KS was a gift from Hiroshi Sasaki (Addgene plasmid # 121974 ; http://n2t.net/addgene:121974 ; RRID:Addgene_121974)

  • For your References section:

    A binding site for Gli proteins is essential for HNF-3beta floor plate enhancer activity in transgenics and can respond to Shh in vitro. Sasaki H, Hui C, Nakafuku M, Kondoh H. Development. 1997 Apr . 124(7):1313-22. PubMed 9118802
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