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Addgene

hGli3 NTOTAL in AINGpCAGGS
(Plasmid #121973)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 121973 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Linker A IRES NLS GFP pCAGGS
  • Backbone manufacturer
    home made using pCAGGS
  • Backbone size w/o insert (bp) 6200
  • Vector type
    Mammalian Expression ; chick electroporation

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hGli3 NTOTAL
  • Alt name
    Gli3R
  • Species
    H. sapiens (human)
  • Mutation
    C terminus deletion from zinc finger domain
  • Entrez Gene
    GLI3 (a.k.a. ACLS, GCPS, GLI3-190, GLI3FL, PAP-A, PAPA, PAPA1, PAPB, PHS, PPDIV)
  • Tags / Fusion Proteins
    • 5xmyc (N terminal on insert)
    • Linker A IRES NLS GFP pCAGGS (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ClaI (not destroyed)
  • 3′ cloning site SacI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The GLI3 insert contains an S1131G difference compared to NM_000168.6 that does not affect function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hGli3 NTOTAL in AINGpCAGGS was a gift from James Briscoe (Addgene plasmid # 121973 ; http://n2t.net/addgene:121973 ; RRID:Addgene_121973)

  • For your References section:

    Inhibitory Gli3 activity negatively regulates Wnt/beta-catenin signaling. Ulloa F, Itasaki N, Briscoe J. Curr Biol. 2007 Mar 20;17(6):545-50. doi: 10.1016/j.cub.2007.01.062. Epub 2007 Mar 1. 10.1016/j.cub.2007.01.062 PubMed 17331723
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