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Addgene

Gli3ZF in AINGpCAGGS
(Plasmid #121972)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 121972 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Linker A IRES NLS GFP pCAGGS
  • Backbone manufacturer
    home made using pCAGGS
  • Backbone size w/o insert (bp) 6200
  • Vector type
    Mammalian Expression ; chick electroporation

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Gli3ZF
  • Alt name
    GliZF
  • Species
    H. sapiens (human)
  • Mutation
    N terminus and C terminus deletion only nt 1411-1935 included
  • Entrez Gene
    GLI3 (a.k.a. ACLS, GCPS, GLI3-190, GLI3FL, PAP-A, PAPA, PAPA1, PAPB, PHS, PPDIV)
  • Tag / Fusion Protein
    • 5xmyc (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ClaI (not destroyed)
  • 3′ cloning site NheI (destroyed during cloning)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The GLI3 insert contains an S1131G difference compared to NM_000168.6 that does not affect function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Gli3ZF in AINGpCAGGS was a gift from James Briscoe (Addgene plasmid # 121972 ; http://n2t.net/addgene:121972 ; RRID:Addgene_121972)
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