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DN2-Gli3 in AINGpCAGGS
(Plasmid #121969)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 121969 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Linker A IRES NLS GFP pCAGGS
  • Backbone manufacturer
    home made using pCAGGS
  • Backbone size w/o insert (bp) 6200
  • Vector type
    Mammalian Expression ; chick electroporation

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DN2Gli3
  • Alt name
    Gli3A^HIGH
  • Species
    H. sapiens (human)
  • Mutation
    N terminus deletion (nt1-1401)
  • Entrez Gene
    GLI3 (a.k.a. ACLS, GCPS, GLI3-190, GLI3FL, PAP-A, PAPA, PAPA1, PAPB, PHS, PPDIV)
  • Tag / Fusion Protein
    • 5xmyc (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (destroyed during cloning)
  • 3′ cloning site NheI (destroyed during cloning)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The GLI3 insert contains an S1131G difference compared to NM_000168.6 that does not affect function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    DN2-Gli3 in AINGpCAGGS was a gift from James Briscoe (Addgene plasmid # 121969 ; http://n2t.net/addgene:121969 ; RRID:Addgene_121969)

  • For your References section:

    A gradient of Gli activity mediates graded Sonic Hedgehog signaling in the neural tube. Stamataki D, Ulloa F, Tsoni SV, Mynett A, Briscoe J. Genes Dev. 2005 Mar 1;19(5):626-41. doi: 10.1101/gad.325905. 10.1101/gad.325905 PubMed 15741323
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