pPUR-mU6-sgRNA-Sirius-8XPP7
(Plasmid #121943)

• Purpose: CRISPR-Sirius plasmid
• Depositing Lab: Thoru Pederson
• Publication: Ma et al Nat Methods. 2018 Nov;15(11):928-931. doi: 10.1038/s41592-018-0174-0. Epub 2018 Oct 30.

Backbone

• Vector backbone: pLKO.1
• Backbone size w/o insert (bp): 8541
• Total vector size (bp): 8961
• Vector type: Mammalian Expression, Lentiviral, CRISPR
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): ccdB Survival
• Growth instructions: May require 2 days to grow
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: sgRNA-Sirius-8XPP7
• Species: Synthetic
• Insert Size (bp): 420
• Promoter: mouse U6 promoter
• Tag / Fusion Protein:
  • no

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Sbf I (not destroyed)
• 3′ cloning site: EcoR I (not destroyed)
• 5′ sequencing primer: gatccgacgccgccatctct
• 3′ sequencing primer: CTATTCTTTCCCCTGCACTGTACCC

Resource Information

• Supplemental Documents:
  • pPUR-mU6-sgRNA-Sirius-8XPP7.gb
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry