pPUR-hU6-sgRNA-Sirius-8XMS2 Citations (4)
Originally described in: CRISPR-Sirius: RNA scaffolds for signal amplification in genome imaging.Ma H, Tu LC, Naseri A, Chung YC, Grunwald D, Zhang S, Pederson T Nat Methods. 2018 Nov;15(11):928-931. doi: 10.1038/s41592-018-0174-0. Epub 2018 Oct 30. PubMed Journal
Articles Citing pPUR-hU6-sgRNA-Sirius-8XMS2
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CRISPR FISHer enables high-sensitivity imaging of nonrepetitive DNA in living cells through phase separation-mediated signal amplification.
Lyu XY, Deng Y, Huang XY, Li ZZ, Fang GQ, Yang D, Wang FL, Kang W, Shen EZ, Song CQ.
Cell Res. 2022 Nov;32(11):969-981. doi: 10.1038/s41422-022-00712-z. Epub 2022 Sep 14.
PubMed
Associated Plasmids |
| Depletion of lamins B1 and B2 promotes chromatin mobility and induces differential gene expression by a mesoscale-motion-dependent mechanism. Pujadas Liwag EM, Wei X, Acosta N, Carter LM, Yang J, Almassalha LM, Jain S, Daneshkhah A, Rao SSP, Seker-Polat F, MacQuarrie KL, Ibarra J, Agrawal V, Aiden EL, Kanemaki MT, Backman V, Adli M. Genome Biol. 2024 Mar 22;25(1):77. doi: 10.1186/s13059-024-03212-y. PubMed |
Modular dual-color BiAD sensors for locus-specific readout of epigenome modifications in single cells.
Kohler AR, Hausser J, Harsch A, Bernhardt S, Haussermann L, Brenner LM, Lungu C, Olayioye MA, Bashtrykov P, Jeltsch A.
Cell Rep Methods. 2024 Mar 26:100739. doi: 10.1016/j.crmeth.2024.100739.
PubMed
Associated Plasmids |
| Formamide denaturation of double-stranded DNA for fluorescence in situ hybridization (FISH) distorts nanoscale chromatin structure. Shim AR, Frederick J, Pujadas EM, Kuo T, Ye IC, Pritchard JA, Dunton CL, Gonzalez PC, Acosta N, Jain S, Anthony NM, Almassalha LM, Szleifer I, Backman V. PLoS One. 2024 May 28;19(5):e0301000. doi: 10.1371/journal.pone.0301000. eCollection 2024. PubMed |
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