pHAGE-TO-dCas9-P2A-HSA
(Plasmid #121936)

• Purpose: CRISPR-Sirius plasmid
• Depositing Lab: Thoru Pederson
• Publication: Ma et al Nat Methods. 2018 Nov;15(11):928-931. doi: 10.1038/s41592-018-0174-0. Epub 2018 Oct 30.

Backbone

• Vector backbone: pHAGE-TO
• Backbone size w/o insert (bp): 6645
• Total vector size (bp): 11108
• Vector type: Mammalian Expression, Lentiviral, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: dCas9-P2A-HSA
• Species: Synthetic
• Insert Size (bp): 4463
• Promoter: CMV-TO
• Tag / Fusion Protein:
  • HSA (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: cgaaggaatagaagaagaaggtgg
• 3′ sequencing primer: CCAAAGGGAGATCCGACTCG

Resource Information

• Supplemental Documents:
  • pHAGE-TO-dCas9-P2A-HSA.gb
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry