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pAAV-hSyn-DIO-GRAB_ACh3.0
(Plasmid #121923)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 121923 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV vector
  • Backbone size w/o insert (bp) 4495
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GPCR activation based ACh sensor GRAB-ACh3.0
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1905
  • Promoter hSyn

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-hSyn-DIO-GRAB_ACh3.0 was a gift from Yulong Li (Addgene plasmid # 121923 ; http://n2t.net/addgene:121923 ; RRID:Addgene_121923)

  • For your References section:

    An optimized acetylcholine sensor for monitoring in vivo cholinergic activity. Jing M, Li Y, Zeng J, Huang P, Skirzewski M, Kljakic O, Peng W, Qian T, Tan K, Zou J, Trinh S, Wu R, Zhang S, Pan S, Hires SA, Xu M, Li H, Saksida LM, Prado VF, Bussey TJ, Prado MAM, Chen L, Cheng H, Li Y. Nat Methods. 2020 Sep 28. pii: 10.1038/s41592-020-0953-2. doi: 10.1038/s41592-020-0953-2. 10.1038/s41592-020-0953-2 PubMed 32989318
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