pHBDuet116
(Plasmid
#121915)
-
PurposeNpuDnaBΔ290ΔC39 intein
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 121915 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepRSFDuet-1
-
Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 3995
- Total vector size (bp) 4289
-
Modifications to backboneHis6-GB1 tag, deletion of MCS-2
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameOth-NpuDnaBΔ290ΔC39 intein
-
SpeciesNostoc punctiforme
-
Insert Size (bp)294
-
MutationDeletion of 290-residue endonuclease domain, I53K, P55K, T58E
-
GenBank IDCP001037.1 ACC81364.1
- Promoter T7
-
Tag
/ Fusion Protein
- His6-GB1 (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer T7 Forward
- 3′ sequencing primer T7 Reverse (Common Sequencing Primers)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pHBDuet116 was a gift from Hideo Iwai (Addgene plasmid # 121915 ; http://n2t.net/addgene:121915 ; RRID:Addgene_121915) -
For your References section:
The crystal structure of the naturally split gp41-1 intein guides the engineering of orthogonal split inteins from cis-splicing inteins. Beyer HM, Mikula KM, Li M, Wlodawer A, Iwai H. FEBS J. 2019 Oct 30. doi: 10.1111/febs.15113. 10.1111/febs.15113 PubMed 31665813