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Addgene

pHBBAD113
(Plasmid #121912)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 121912 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBAD
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 4798
  • Total vector size (bp) 4918
  • Modifications to backbone
    GB1-His6 tag
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    CI-NpuDnaBC39 intein
  • Species
    Nostoc punctiforme
  • Insert Size (bp)
    120
  • Mutation
    S110K, I111K
  • GenBank ID
    CP001037.1 ACC81364.1
  • Promoter Para
  • Tag / Fusion Protein
    • GB1-His6 (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHBBAD113 was a gift from Hideo Iwai (Addgene plasmid # 121912 ; http://n2t.net/addgene:121912 ; RRID:Addgene_121912)

  • For your References section:

    The crystal structure of the naturally split gp41-1 intein guides the engineering of orthogonal split inteins from cis-splicing inteins. Beyer HM, Mikula KM, Li M, Wlodawer A, Iwai H. FEBS J. 2019 Oct 30. doi: 10.1111/febs.15113. 10.1111/febs.15113 PubMed 31665813
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