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Addgene

pSADuet148
(Plasmid #121910)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 121910 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRSFDuet-1
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 3994
  • Total vector size (bp) 4289
  • Modifications to backbone
    His6-GB1 tag, deletion of MCS-2
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CI-NpuDnaBΔ290ΔC39 intein
  • Species
    Nostoc punctiforme
  • Insert Size (bp)
    295
  • Mutation
    Deletion of 290-residue endonuclease domain, I53K, P55K, E58K
  • GenBank ID
    CP001037.1 ACC81364.1
  • Promoter T7
  • Tag / Fusion Protein
    • His6-GB1 (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site HindIII (unknown if destroyed)
  • 5′ sequencing primer T7 Forward
  • 3′ sequencing primer T7 Reverse
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSADuet148 was a gift from Hideo Iwai (Addgene plasmid # 121910 ; http://n2t.net/addgene:121910 ; RRID:Addgene_121910)

  • For your References section:

    The crystal structure of the naturally split gp41-1 intein guides the engineering of orthogonal split inteins from cis-splicing inteins. Beyer HM, Mikula KM, Li M, Wlodawer A, Iwai H. FEBS J. 2019 Oct 30. doi: 10.1111/febs.15113. 10.1111/febs.15113 PubMed 31665813
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