p3xFlag-Dam
(Plasmid
#121909)
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PurposeExpresses E. coli Dam methyltransferase containing an N-ternimal 3xFlag tag
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 121909 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonep3Flag-EYFP
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Backbone manufacturerSigma
- Backbone size w/o insert (bp) 5400
- Total vector size (bp) 6231
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameDam human codon optimized
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SpeciesE. coli
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Insert Size (bp)831
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GenBank ID
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Entrez Genedam (a.k.a. b3387, ECK3374)
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Tag
/ Fusion Protein
- Flag (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p3xFlag-Dam was a gift from Douglas Feldman (Addgene plasmid # 121909 ; http://n2t.net/addgene:121909 ; RRID:Addgene_121909) -
For your References section:
An Adversarial DNA N(6)-Methyladenine-Sensor Network Preserves Polycomb Silencing. Kweon SM, Chen Y, Moon E, Kvederaviciute K, Klimasauskas S, Feldman DE. Mol Cell. 2019 Apr 5. pii: S1097-2765(19)30222-9. doi: 10.1016/j.molcel.2019.03.018. 10.1016/j.molcel.2019.03.018 PubMed 30982744
