pBAD-BirA-eCPX-AP(K10)
(Plasmid #121908)

• Purpose: Expresses BirA and eCPX-AP(K10)
• Depositing Lab: Per Svenningsen
• Publication: Granhoj et al Sci Rep. 2019 Mar 11;9(1):4118. doi: 10.1038/s41598-019-40984-x.

Backbone

• Vector backbone: pBAD
• Backbone size w/o insert (bp): 4000
• Total vector size (bp): 5919
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: BirA
• Species: Synthetic
• Insert Size (bp): 984
• Entrez Gene: birA (a.k.a. b3973, ECK3965, bioR, dhbB)
• Promoter: pBAD promoter
• Tag / Fusion Protein:
  • 6xHis (C terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: pBAD

Gene/Insert 2

• Gene/Insert name: enhanced Circularly Permuted Outer Membrane Protein X
• Alt name: eCPX
• Species: Synthetic
• Insert Size (bp): 627
• Mutation: changed lysine 10 in the AP to alanine
• Promoter: T7

Cloning Information for Gene/Insert 2

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: T7

Resource Information

• Supplemental Documents:
  • BirA insert eCPX K to A.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry