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Addgene

PCRII apoEa
(Plasmid #121900)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 121900 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCRII
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 3931
  • Total vector size (bp) 4739
  • Vector type
    Unspecified

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Kanamycin, 100 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ApoEa
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    768
  • Entrez Gene
    apoea (a.k.a. im:7036787, wu:fb69a05, zgc:110064)

Cloning Information

  • Cloning method TOPO Cloning
  • 5′ sequencing primer GAA CGA CTG CGA TCC AAG C
  • 3′ sequencing primer GAG CTC CTG CAT TCG TGA GT
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PCRII apoEa was a gift from Steven Farber (Addgene plasmid # 121900 ; http://n2t.net/addgene:121900 ; RRID:Addgene_121900)

  • For your References section:

    Zebrafish as a model for apolipoprotein biology: comprehensive expression analysis and a role for ApoA-IV in regulating food intake. Otis JP, Zeituni EM, Thierer JH, Anderson JL, Brown AC, Boehm ED, Cerchione DM, Ceasrine AM, Avraham-Davidi I, Tempelhof H, Yaniv K, Farber SA. Dis Model Mech. 2015 Mar;8(3):295-309. doi: 10.1242/dmm.018754. Epub 2015 Jan 29. 10.1242/dmm.018754 PubMed 25633982
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