pCMV5 MEKK4alpha KD
(Plasmid #12188)

• Depositing Lab: Gary Johnson
• Publication: Gerwins et al J Biol Chem. 1997 Mar 28. 272(13):8288-95.

Backbone

• Vector backbone: pCMV5
• Backbone size w/o insert (bp): 4600
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MEKK4
• Alt name: MEKK4 alpha
• Species: M. musculus (mouse)
• Insert Size (bp): 4900
• Mutation: Kinase dead: K1361M
• GenBank ID: U85607
• Entrez Gene: Map3k4 (a.k.a. D17Rp17, D17Rp17e, MAPKKK4, MEKK 4, MTK1, Mek4b, Mekk4, RP17, Rp17a, Tas, mKIAA0213)
• Tag / Fusion Protein:
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (not destroyed)
• 3′ cloning site: ApaI (destroyed during cloning)
• 5′ sequencing primer: CMV-F

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry