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Addgene

JN302: pMVP/SB/Puro-DEST
(Plasmid #121865)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 121865 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    JN607
  • Backbone manufacturer
    Newgard Lab
  • Backbone size (bp) 4462
  • Vector type
    Mammalian Expression ; Sleeping Beauty Transposon, pMVP Gateway Destination Vector
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Gateway selection cassette (ccdb gene + Chlor resistance gene)
  • Species
    Synthetic
  • Insert Size (bp)
    1455

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Sleeping Beauty ITR sequences derived from Addgene #26562

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    JN302: pMVP/SB/Puro-DEST was a gift from Christopher Newgard (Addgene plasmid # 121865 ; http://n2t.net/addgene:121865 ; RRID:Addgene_121865)

  • For your References section:

    Creation of versatile cloning platforms for transgene expression and dCas9-based epigenome editing. Haldeman JM, Conway AE, Arlotto ME, Slentz DH, Muoio DM, Becker TC, Newgard CB. Nucleic Acids Res. 2018 Dec 27. pii: 5264291. doi: 10.1093/nar/gky1286. 10.1093/nar/gky1286 PubMed 30590691
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