pMSCV MEKK3 PB1
(Plasmid
#12185)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 12185 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepMSCVpuro
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 6300
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Vector typeMammalian Expression, Retroviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMEKK3
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SpeciesM. musculus (mouse)
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Insert Size (bp)300
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MutationPB1 domain: aa 36-127
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GenBank IDNM_011947
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Entrez GeneMap3k3 (a.k.a. AW548911, MAPKKK3, Mekk3, mKIAA4031)
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Tag
/ Fusion Protein
- Flag (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer pMSCV 5'
- 3′ sequencing primer pMSCV 3' (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMSCV MEKK3 PB1 was a gift from Gary Johnson (Addgene plasmid # 12185 ; http://n2t.net/addgene:12185 ; RRID:Addgene_12185) -
For your References section:
PB1 domains of MEKK2 and MEKK3 interact with the MEK5 PB1 domain for activation of the ERK5 pathway. Nakamura K, Johnson GL. J Biol Chem. 2003 Sep 26. 278(39):36989-92. 10.1074/jbc.C300313200 PubMed 12912994