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KK701: pMAGIC (L3-L2) 3x HA eptitope tag + polyA; hU6::xCas9(3.7) gRNA scaffold
(Plasmid #121842)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 121842 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDONR221 P3-P2
  • Backbone manufacturer
    Invitrogen
  • Backbone size (bp) 2555
  • Vector type
    Synthetic Biology ; pMAGIC Gateway Entry Plasmid

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    3x HA epitope tag-PolyA + hU6-xCas9(3.7) gRNA scaffold
  • Alt name
    3x HA epitope tag-polyA; hU6::SpCas9 gRNA scaffold
  • Species
    Synthetic
  • Insert Size (bp)
    711

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Annealed oligonucleotides containing the protospacer motif can be ligated into BsaI digested plasmid. 3x HA tag + bGH polyA; hU6 promoter PCR'd from Addgene 61591. x/SpCas9 gRNA scaffold derived from PMID: 26671237

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    KK701: pMAGIC (L3-L2) 3x HA eptitope tag + polyA; hU6::xCas9(3.7) gRNA scaffold was a gift from Christopher Newgard (Addgene plasmid # 121842 ; http://n2t.net/addgene:121842 ; RRID:Addgene_121842)

  • For your References section:

    Creation of versatile cloning platforms for transgene expression and dCas9-based epigenome editing. Haldeman JM, Conway AE, Arlotto ME, Slentz DH, Muoio DM, Becker TC, Newgard CB. Nucleic Acids Res. 2018 Dec 27. pii: 5264291. doi: 10.1093/nar/gky1286. 10.1093/nar/gky1286 PubMed 30590691
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