pcDNA3 MEKK1 KD
(Plasmid #12180)

• Depositing Lab: Gary Johnson
• Publication: Widmann et al Mol Cell Biol. 1998 Apr . 18(4):2416-29.

Backbone

• Vector backbone: pcDNA3.1/Hygro
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5600
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MEKK1
• Species: M. musculus (mouse)
• Insert Size (bp): 5200
• Mutation: Kinase dead: K1253M; V1467L
• GenBank ID: AF117340
• Entrez Gene: Map3k1 (a.k.a. MAPKKK1, MEKK1, Mekk)
• Tags / Fusion Proteins:
  • His (N terminal on insert)
  • Xpress (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: T7

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that Addgene's sequencing results identified a V1467L mutation in the MEKK1 sequence when compared to GenBank ID AF117340.1. The depositing laboratory states that this plasmid functions exactly as intended and the mutation may represent a valid polymorphism.