pSKDuet01 Citations (20)
Originally described in: Highly efficient protein trans-splicing by a naturally split DnaE intein from Nostoc punctiforme.Iwai H, Zuger S, Jin J, Tam PH FEBS Lett. 2006 Mar 20. 580(7):1853-8. PubMed Journal
Articles Citing pSKDuet01
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Segmental isotopic labeling of multi-domain and fusion proteins by protein trans-splicing in vivo and in vitro.
Muona M, Aranko AS, Raulinaitis V, Iwaï H.
Nat Protoc. 2010 . 5(3):574-87.
PubMed
Associated Plasmids |
Split-intein mediated re-assembly of genetically encoded Ca(2+) indicators.
Wong SS, Kotera I, Mills E, Suzuki H, Truong K.
Cell Calcium. 2011 Nov 29.
PubMed
Associated Plasmids |
| Simultaneous assembly of two target proteins using split inteins for live cell imaging. Wong S, Mills E, Truong K. Protein Eng Des Sel. 2013 Mar;26(3):207-13. doi: 10.1093/protein/gzs100. Epub 2012 Dec 4. PubMed |
| Parts-based assembly of synthetic transmembrane proteins in mammalian cells. Nagaraj S, Wong S, Truong K. ACS Synth Biol. 2012 Apr 20;1(4):111-7. doi: 10.1021/sb200007r. Epub 2011 Nov 11. PubMed |
| A general purification platform for toxic proteins based on intein trans-splicing. Shi C, Tarimala A, Meng Q, Wood DW. Appl Microbiol Biotechnol. 2014 Nov;98(22):9425-35. doi: 10.1007/s00253-014-6080-1. Epub 2014 Oct 9. PubMed |
| A dual-intein autoprocessing domain that directs synchronized protein co-expression in both prokaryotes and eukaryotes. Zhang B, Rapolu M, Liang Z, Han Z, Williams PG, Su WW. Sci Rep. 2015 Feb 25;5:8541. doi: 10.1038/srep08541. PubMed |
| An Engineered Split Intein for Photoactivated Protein Trans-Splicing. Wong S, Mosabbir AA, Truong K. PLoS One. 2015 Aug 28;10(8):e0135965. doi: 10.1371/journal.pone.0135965. eCollection 2015. PubMed |
| Locked and proteolysis-based transcription activator-like effector (TALE) regulation. Lonzaric J, Lebar T, Majerle A, Mancek-Keber M, Jerala R. Nucleic Acids Res. 2016 Jan 8. pii: gkv1541. PubMed |
| Generation of camelid VHH bispecific constructs via in-cell intein-mediated protein trans-splicing. Shibuya Y, Haga N, Asano R, Nakazawa H, Hattori T, Takeda D, Sugiyama A, Kurotani R, Kumagai I, Umetsu M, Makabe K. Protein Eng Des Sel. 2017 Jan;30(1):15-21. Epub 2016 Nov 23. PubMed |
| Intein-mediated assembly of tunable scaffoldins for facile synthesis of designer cellulosomes. Han Z, Su WW. Appl Microbiol Biotechnol. 2018 Feb;102(3):1331-1342. doi: 10.1007/s00253-017-8701-y. Epub 2017 Dec 23. PubMed |
| Intein-mediated backbone cyclization of entolimod confers enhanced radioprotective activity in mouse models. Ye B, Shen W, Shi M, Zhang Y, Xu C, Zhao Z. PeerJ. 2018 Jun 18;6:e5043. doi: 10.7717/peerj.5043. eCollection 2018. PubMed |
Design of fast proteolysis-based signaling and logic circuits in mammalian cells.
Fink T, Lonzaric J, Praznik A, Plaper T, Merljak E, Leben K, Jerala N, Lebar T, Strmsek Z, Lapenta F, Bencina M, Jerala R.
Nat Chem Biol. 2019 Feb;15(2):115-122. doi: 10.1038/s41589-018-0181-6. Epub 2018 Dec 10.
PubMed
Associated Plasmids |
| Intein-ubiquitin chimeric domain for coordinated protein coexpression. Zhang B, Han Z, Kumar S, Gupta M, Su WW. J Biotechnol. 2019 Oct 10;304:38-43. doi: 10.1016/j.jbiotec.2019.08.006. Epub 2019 Aug 12. PubMed |
| Smad7 Binds Differently to Individual and Tandem WW3 and WW4 Domains of WWP2 Ubiquitin Ligase Isoforms. Wahl LC, Watt JE, Yim HTT, De Bourcier D, Tolchard J, Soond SM, Blumenschein TMA, Chantry A. Int J Mol Sci. 2019 Sep 21;20(19). pii: ijms20194682. doi: 10.3390/ijms20194682. PubMed |
The crystal structure of the naturally split gp41-1 intein guides the engineering of orthogonal split inteins from cis-splicing inteins.
Beyer HM, Mikula KM, Li M, Wlodawer A, Iwai H.
FEBS J. 2019 Oct 30. doi: 10.1111/febs.15113.
PubMed
Associated Plasmids |
| Quantification, regulation and production of 5-aminolevulinic acid by green fluorescent protein in recombinant Escherichia coli. Tan SI, You SC, Shih IT, Ng IS. J Biosci Bioeng. 2019 Oct 30. pii: S1389-1723(19)30846-1. doi: 10.1016/j.jbiosc.2019.10.005. PubMed |
Metabolic enzyme clustering by coiled coils improves the biosynthesis of resveratrol and mevalonate.
Fink T, Stevovic B, Verwaal R, Roubos JA, Gaber R, Bencina M, Jerala R, Gradisar H.
AMB Express. 2020 May 24;10(1):97. doi: 10.1186/s13568-020-01031-5.
PubMed
Associated Plasmids |
| Dengue virus protease activity modulated by dynamics of protease cofactor. Lee WHK, Liu W, Fan JS, Yang D. Biophys J. 2021 Jun 15;120(12):2444-2453. doi: 10.1016/j.bpj.2021.04.015. Epub 2021 Apr 22. PubMed |
| The NMR structure of the engineered halophilic DnaE intein for segmental isotopic labeling using conditional protein splicing. Heikkinen HA, Aranko AS, Iwai H. J Magn Reson. 2022 May;338:107195. doi: 10.1016/j.jmr.2022.107195. Epub 2022 Mar 18. PubMed |
| Ligation of multiple protein domains using orthogonal inteins with non-native splice junctions. Romero-Casanas A, Garcia-Lizarribar A, Castro J, Vilanova M, Benito A, Ribo M. Protein Sci. 2024 Jul;33(7):e5070. doi: 10.1002/pro.5070. PubMed |
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