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Addgene

MB121 (Nf2 BBA)
(Plasmid #12170)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 12170 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pIRES-hrGFP II
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Nf2 BBA
  • Alt name
    Nf2
  • Species
    M. musculus (mouse)
  • Mutation
    BBA: dominant negative Nf2, 7 Blue Box residues changed to alanine
  • Entrez Gene
    Nf2 (a.k.a. merlin)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MB121 (Nf2 BBA) was a gift from Tyler Jacks (Addgene plasmid # 12170 ; http://n2t.net/addgene:12170 ; RRID:Addgene_12170)
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