pHGPD/P82
(Plasmid
#1217)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 1217 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRS314
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Backbone manufactureratcc
- Backbone size w/o insert (bp) 8600
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Vector typeYeast Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namehsp82
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Alt namehsp82
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Alt namehsp90
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Alt nameheat shock
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)3100
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MutationGPD promoter [pHGPD] marked plasmid, select for with HIS3
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GenBank IDNC_001148
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Entrez GeneHSP82 (a.k.a. YPL240C, HSP90)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHGPD/P82 was a gift from Susan Lindquist (Addgene plasmid # 1217 ; http://n2t.net/addgene:1217 ; RRID:Addgene_1217) -
For your References section:
Mutational analysis of Hsp90 function: interactions with a steroid receptor and a protein kinase. Nathan DF, Lindquist S. Mol Cell Biol 1995 Jul;15(7):3917-25. PubMed 7791797