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pRG/III-hs-MBP-CB2-HF
(Plasmid #121682)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 121682 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRG/III-hs-MBP
  • Backbone size w/o insert (bp) 6991
  • Total vector size (bp) 8141
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    peripheral cannabinoid receptor
  • Alt name
    CB2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1092
  • Entrez Gene
    CNR2 (a.k.a. CB-2, CB2, CX5)
  • Promoter lac
  • Tag / Fusion Protein
    • His6 Flag tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer MalE2
  • 3′ sequencing primer M13 forward
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Internal plasmid code # 611

This plasmid is in fact published: Calandra 1997, see uploaded paper. The citation however does not show in PubMed.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRG/III-hs-MBP-CB2-HF was a gift from Reinhard Grisshammer & Richard Henderson (Addgene plasmid # 121682 ; http://n2t.net/addgene:121682 ; RRID:Addgene_121682)

  • For your References section:

    Expression in Escherichia coli and characterisation of the human central CB1 and peripheral CB2 cannabinoid receptors. Calandra Bernard, Julie Tucker, David Shire, Reinhard Grisshammer. Biotechnology Letters 10.1023/A:1018383808401
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