pMB80 (R26-CreER)
(Plasmid #12168)

• Depositing Lab: Tyler Jacks
• Publication: McLaughlin et al Proc Natl Acad Sci U S A. 2007 Feb 27. 104(9):3261-6.

Backbone

• Vector backbone: pROSA26-1
• Backbone manufacturer: P. Soriano
• Vector type: Mammalian Expression, Cre/Lox
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Rosa26 CreER^T2
• Alt name: CreER

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: na

Resource Information

• Addgene Notes:
  • Addgene diagnostic digest
• A portion of this plasmid was derived from a plasmid made by: CreER^T2 cDNA from P. Chambon (Indra et al., 1999)
• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Targeting vector to generate R26-CreER^T2 mice. XbaI/MluI SA-CreER^T2 fragment of pMB61, MluI/XbaI PA-floxed PGK-Puro fragment of pMB75, and XbaI-digested modified version of pROSA26-1 vector where SacII site is replaced by an AscI linearization site.

See sequence link for sequence of insert from author. Please note that there are minor errors in the sequence, specifically there are no XbaI sites within the insert. Please see Addgene test digest with XbaI for the expected bands.