pRG/III-hs-MBP
(Plasmid
#121676)
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PurposeBacterial expression of MBP fusion proteins
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Depositing Labs
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 121676 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRG/III-hs-MBP
- Total vector size (bp) 6991
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namemaltose binding protein
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Alt nameMBP
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SpeciesE. coli
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Insert Size (bp)1176
- Promoter lac promoter
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer M13 reverse
- 3′ sequencing primer M13 forward (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
pRG/III-hs-MBP is a derivative of NEB's pMal-p2 vector, see Tucker Grisshammer Biochemical Journal 317, 891, 1996.
Internal plasmid code # 591
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRG/III-hs-MBP was a gift from Reinhard Grisshammer & Richard Henderson (Addgene plasmid # 121676 ; http://n2t.net/addgene:121676 ; RRID:Addgene_121676) -
For your References section:
Purification of a rat neurotensin receptor expressed in Escherichia coli. Tucker J, Grisshammer R. Biochem J. 1996 Aug 1;317 ( Pt 3):891-9. PubMed 8760379
