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Addgene

pCF730
(Plasmid #121668)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 121668 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEF1a
  • Backbone size w/o insert (bp) 11094
  • Total vector size (bp) 13593
  • Vector type
    Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    one more resistance cassette: puroR
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cas9(C)
  • Alt name
    c terminal portion of cas9
  • Species
    Streptococcus pyogenes
  • Insert Size (bp)
    2499
  • Promoter EF-1a core promoter

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer 5'-AATGAGAAAGCCCGCCTTCC-3'
  • 3′ sequencing primer 5'-CTCCGCGTTTCTTTTGTTTGTCG-3'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCF730 was a gift from David Savage (Addgene plasmid # 121668 ; http://n2t.net/addgene:121668 ; RRID:Addgene_121668)

  • For your References section:

    CRISPR-Cas9 Circular Permutants as Programmable Scaffolds for Genome Modification. Oakes BL, Fellmann C, Rishi H, Taylor KL, Ren SM, Nadler DC, Yokoo R, Arkin AP, Doudna JA, Savage DF. Cell. 2019 Jan 10;176(1-2):254-267.e16. doi: 10.1016/j.cell.2018.11.052. 10.1016/j.cell.2018.11.052 PubMed 30633905
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