pDEST-APIC-Cas9
(Plasmid
#121657)
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Purpose(Empty Backbone) gateway cloning destination vector for expressing enhancer driven Cas9
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 121657 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAPIC
- Backbone size (bp) 9163
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
For more information about the Han Lab Drosophila Transgenic Vectors, please visit: https://han.wicmb.cornell.edu/han-lab-drosophila-transgenic-vectors/
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDEST-APIC-Cas9 was a gift from Chun Han (Addgene plasmid # 121657 ; http://n2t.net/addgene:121657 ; RRID:Addgene_121657) -
For your References section:
Robust CRISPR/Cas9-Mediated Tissue Specific Mutagenesis Reveals Gene Redundancy and Perdurance in Drosophila. Poe AR, Wang B, Sapar ML, Ji H, Li K, Onabajo T, Fazliyeva R, Gibbs M, Qiu Y, Hu Y, Han C. Genetics. 2018 Nov 30. pii: genetics.118.301736. doi: 10.1534/genetics.118.301736. 10.1534/genetics.118.301736 PubMed 30504366