pSico Dnmt1
(Plasmid #12165)

• Depositing Lab: Tyler Jacks
• Publication: Ventura et al Proc Natl Acad Sci U S A. 2004 Jul 13. 101(28):10380-5.

Backbone

• Vector backbone: pSico
• Backbone size w/o insert (bp): 7560
• Vector type: Mammalian Expression, Lentiviral, RNAi, Cre/Lox

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Dnmt1 shRNA
• Alt name: Dnmt1
• Species: M. musculus (mouse)
• Insert Size (bp): 55
• Mutation: EGFP is expressed from this plasmid as a marker, but it is not a fusion protein. Cre causes EGFP to be recombined out of the construct, activating Dnmt1 shRNA expression.
• Entrez Gene: Dnmt1 (a.k.a. Cxxc9, Dnmt, Dnmt1o, MCMT, MTase, Met-1, Met1, MommeD2, m.MmuI)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HpaI (destroyed during cloning)
• 3′ cloning site: XhoI (destroyed during cloning)
• 5′ sequencing primer: EGFP-C

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Cre TURNS ON Dmnt1 shRNA expression. The shRNA is directed against the mouse Dnmt1 sequence 5'-GAGTGTGTGAGGGAGAAA-3'.