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Addgene

pBLO44.1_dProtease
(Plasmid #121631)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 121631 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC
  • Backbone size w/o insert (bp) 4660
  • Total vector size (bp) 5368
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TEV protease
  • Species
    tobacco etch virus
  • Insert Size (bp)
    708
  • Promoter chicken beta-actin promoter

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer 5-'TGGCCAGCggagaaagtttg-3'
  • 3′ sequencing primer 5'-ACTAGTattcatgagttgagtcgcttccttaa-3'
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Note: Addgene NGS unable to fully resolve CAG promoter sequence. Please refer to depositor's sequence for this section of the plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBLO44.1_dProtease was a gift from David Savage (Addgene plasmid # 121631 ; http://n2t.net/addgene:121631 ; RRID:Addgene_121631)

  • For your References section:

    Profiling of engineering hotspots identifies an allosteric CRISPR-Cas9 switch. Oakes BL, Nadler DC, Flamholz A, Fellmann C, Staahl BT, Doudna JA, Savage DF. Nat Biotechnol. 2016 May 2. doi: 10.1038/nbt.3528. 10.1038/nbt.3528 PubMed 27136077
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