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Addgene

pLS-CG
(Plasmid #12161)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 12161 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLS
  • Backbone manufacturer
    Verma Lab
  • Backbone size w/o insert (bp) 6943
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    CMV-GFP
  • Alt name
    CMV GFP
  • Alt name
    GFP
  • Insert Size (bp)
    1376

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pLS has a 133-bp deletion in the 3' LTR, generating a self-inactivating (SIN) virus. See article for cloning details.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLS-CG was a gift from Inder Verma (Addgene plasmid # 12161 ; http://n2t.net/addgene:12161 ; RRID:Addgene_12161)

  • For your References section:

    Development of a self-inactivating lentivirus vector. Miyoshi H, Blomer U, Takahashi M, Gage FH, Verma IM. J Virol. 1998 Oct . 72(10):8150-7. PubMed 9733856
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