mCherry pBSDONR P4r-P2
(Plasmid
#121528)
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PurposeFor making C-terminal mCherry fusions using multisite Gateway cloning
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 121528 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBlueScript
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Backbone manufacturerStratagene
- Backbone size w/o insert (bp) 2900
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemCherry
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SpeciesDiscosoma sp.
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Contains mCherry and Gateway Att sequences.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mCherry pBSDONR P4r-P2 was a gift from Roger Innes (Addgene plasmid # 121528 ; http://n2t.net/addgene:121528 ; RRID:Addgene_121528) -
For your References section:
The KEEP ON GOING protein of Arabidopsis recruits the ENHANCED DISEASE RESISTANCE1 protein to trans-Golgi network/early endosome vesicles. Gu Y, Innes RW. Plant Physiol. 2011 Apr;155(4):1827-38. doi: 10.1104/pp.110.171785. Epub 2011 Feb 22. 10.1104/pp.110.171785 PubMed 21343429