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pLHCX-gpASNase1
(Plasmid #121526)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 121526 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLHCX
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 6813
  • Total vector size (bp) 8500
  • Vector type
    Mammalian Expression, Bacterial Expression, Retroviral
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    gpASNase1
  • Alt name
    guinea pig asparaginase 1
  • Alt name
    ASPG
  • Species
    Cavia porcellus (guinea pig)
  • Insert Size (bp)
    1727
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HINDIII (not destroyed)
  • 3′ cloning site CLAI (not destroyed)
  • 5′ sequencing primer 5' pLNCX Seq Primer
  • 3′ sequencing primer 3' pLNCX Seq Primer
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLHCX-gpASNase1 was a gift from Lucas Sullivan & Matthew Vander Heiden (Addgene plasmid # 121526 ; http://n2t.net/addgene:121526 ; RRID:Addgene_121526)

  • For your References section:

    Aspartate is an endogenous metabolic limitation for tumour growth. Sullivan LB, Luengo A, Danai LV, Bush LN, Diehl FF, Hosios AM, Lau AN, Elmiligy S, Malstrom S, Lewis CA, Vander Heiden MG. Nat Cell Biol. 2018 Jul;20(7):782-788. doi: 10.1038/s41556-018-0125-0. Epub 2018 Jun 25. 10.1038/s41556-018-0125-0 [pii] PubMed 29941931
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