pTriEx-RhoA FLARE.sc Biosensor T19N
(Plasmid #12152)

• Depositing Lab: Klaus Hahn
• Publication: Pertz et al Nature. 2006 Apr 20. 440(7087):1069-72.

Backbone

• Vector backbone: pTriEx
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5000
• Vector type: Mammalian Expression, Bacterial Expression, Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: RhoA Biosensor T19N
• Alt name: 6xHis-RBD-CFP-Linker-Citrine-RhoA T19N
• Alt name: RhoA
• Species: H. sapiens (human)
• Insert Size (bp): 2400
• Mutation: RhoA T19N (dominant-negative mutation)*
• Entrez Gene: RHOA (a.k.a. ARH12, ARHA, EDFAOB, RHO12, RHOH12)
• Tag / Fusion Protein:
  • 6xHis (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: TriExUP

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Protocols for production and use of Hahn lab biosensors and photactivatable proteins can be found on their web page, at the URL below. If you have suggestions for the protocols or have any questions, please feel free to contact the Hahn lab. Good luck with your experiments!

Hahn lab biosensor protocol: http://www.hahnlab.com/tools/index.html

Please reference plasmid 12150 pTRIEX-RhoA Biosensor WT for complete sequence of biosensor

Please note: ECFP sequence contains K27R and N165H amino acid changes

*There is also a previously unreported A88T mutation in this plasmid. It is not known to affect function.