pDL278_P23-DsRed-Express2
(Plasmid #121505)

• Purpose: E. coli-streptococcal shuttle vector that expresses rfp
• Depositing Lab: Robert Shields
• Publication: Shields et al Appl Environ Microbiol. 2019 May 17. pii: AEM.00620-19. doi: 10.1128/AEM.00620-19.

Backbone

• Vector backbone: pDL278
• Backbone size w/o insert (bp): 6700
• Total vector size (bp): 7462
• Vector type: E. coli streptococcal shuttle vector

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: For E. coli, grow in Lysogeny broth (LB) containing 50μg/mL Spectinomycin. For S. mutans, select at 1000μg/mL on plates. Other streptococci may require selection on lower amounts of spectinomycin (e.g. 500μg/mL)
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: DsRed-Express2
• Species: Synthetic
• Insert Size (bp): 830
• GenBank ID: MK301207
• Promoter: P23

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SacI (unknown if destroyed)
• 3′ cloning site: SphI (unknown if destroyed)
• 5′ sequencing primer: M13_pUC_rev
• 3′ sequencing primer: M13_pUC_fwd; pBAD_rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry