Skip to main content
Addgene

pGIPz-PD-L1 3SA
(Plasmid #121490)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 121490 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pGIPz
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    PD-L1 3SA
  • Alt name
    B7H1
  • Species
    H. sapiens (human)
  • Mutation
    S176A, T180A, S184A
  • Promoter CMV
  • Tag / Fusion Protein
    • Flag (C terminal on insert)

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site NA (unknown if destroyed)
  • 3′ cloning site NA (unknown if destroyed)
  • 5′ sequencing primer CMV-5'
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    humun PD-L1 shRNA
  • Alt name
    sequence “TTGACTCCATCTTTCTTCA” (Dharmcon V3LHS_401968)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGIPz-PD-L1 3SA was a gift from Mien-Chie Hung (Addgene plasmid # 121490 ; http://n2t.net/addgene:121490 ; RRID:Addgene_121490)

  • For your References section:

    Glycosylation and stabilization of programmed death ligand-1 suppresses T-cell activity. Li CW, Lim SO, Xia W, Lee HH, Chan LC, Kuo CW, Khoo KH, Chang SS, Cha JH, Kim T, Hsu JL, Wu Y, Hsu JM, Yamaguchi H, Ding Q, Wang Y, Yao J, Lee CC, Wu HJ, Sahin AA, Allison JP, Yu D, Hortobagyi GN, Hung MC. Nat Commun. 2016 Aug 30;7:12632. doi: 10.1038/ncomms12632. 10.1038/ncomms12632 PubMed 27572267
Related items:
Commonly requested with: