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pGIPz-PD-L1 4NQ
(Plasmid #121487)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 121487 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGIPz
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    PD-L1 4NQ
  • Alt name
    also contains hPD-L1 shRNA "TTGACTCCATCTTTCTTCA" (Dharmcon V3LHS_401968)
  • Species
    H. sapiens (human)
  • Mutation
    N35Q, N192Q, N200Q and N219Q
  • Promoter CMV
  • Tag / Fusion Protein
    • Flag (C terminal on insert)

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site NA (unknown if destroyed)
  • 3′ cloning site NA (unknown if destroyed)
  • 5′ sequencing primer CMV-5'
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    hPD-L1 shRNA
  • Alt name
    sequence: "TTGACTCCATCTTTCTTCA" (Dharmcon V3LHS_401968)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGIPz-PD-L1 4NQ was a gift from Mien-Chie Hung (Addgene plasmid # 121487 ; http://n2t.net/addgene:121487 ; RRID:Addgene_121487)

  • For your References section:

    Glycosylation and stabilization of programmed death ligand-1 suppresses T-cell activity. Li CW, Lim SO, Xia W, Lee HH, Chan LC, Kuo CW, Khoo KH, Chang SS, Cha JH, Kim T, Hsu JL, Wu Y, Hsu JM, Yamaguchi H, Ding Q, Wang Y, Yao J, Lee CC, Wu HJ, Sahin AA, Allison JP, Yu D, Hortobagyi GN, Hung MC. Nat Commun. 2016 Aug 30;7:12632. doi: 10.1038/ncomms12632. 10.1038/ncomms12632 PubMed 27572267
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