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Addgene

pTJK517
(Plasmid #121474)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 121474 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS314
  • Backbone size w/o insert (bp) 6362
  • Vector type
    Yeast CEN vector
  • Selectable markers
    TRP1

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CNA1
  • Alt name
    CMP1
  • Alt name
    YLR433C
  • Species
    S. cerevisiae (budding yeast)
  • GenBank ID
    NM_001182321
  • Promoter Endogenous genomic promoter region

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (not destroyed)
  • 3′ cloning site Xho1 (not destroyed)
  • 5′ sequencing primer T7 promoter primer
  • 3′ sequencing primer T3 promoter primer
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTJK517 was a gift from Tami Kingsbury (Addgene plasmid # 121474 ; http://n2t.net/addgene:121474 ; RRID:Addgene_121474)

  • For your References section:

    Calcineurin Regulatory Subunit Calcium-Binding Domains Differentially Contribute to Calcineurin Signaling in Saccharomyces cerevisiae. Connolly S, Quasi-Woode D, Waldron L, Eberly C, Waters K, Muller EM, Kingsbury TJ. Genetics. 2018 Jul;209(3):801-813. doi: 10.1534/genetics.118.300911. Epub 2018 May 7. 10.1534/genetics.118.300911 PubMed 29735720
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