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Addgene

pTJK516
(Plasmid #121473)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 121473 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS315
  • Backbone size w/o insert (bp) 6018
  • Vector type
    Yeast CEN plasmid
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CNB1mutEF4
  • Species
    S. cerevisiae (budding yeast)
  • Mutation
    Changed aspartic acid 144 to alanine
  • GenBank ID
    NM_001179756
  • Entrez Gene
    CNB1 (a.k.a. YKL190W, CRV1, YCN2)
  • Promoter Endogenous genomic promoter region

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T3 promoter primer
  • 3′ sequencing primer T7 promoter primer
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTJK516 was a gift from Tami Kingsbury (Addgene plasmid # 121473 ; http://n2t.net/addgene:121473 ; RRID:Addgene_121473)

  • For your References section:

    Calcineurin Regulatory Subunit Calcium-Binding Domains Differentially Contribute to Calcineurin Signaling in Saccharomyces cerevisiae. Connolly S, Quasi-Woode D, Waldron L, Eberly C, Waters K, Muller EM, Kingsbury TJ. Genetics. 2018 Jul;209(3):801-813. doi: 10.1534/genetics.118.300911. Epub 2018 May 7. 10.1534/genetics.118.300911 PubMed 29735720
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