pTJK509
(Plasmid
#121464)
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Purpose2µm HIS3 CNB1mutEF2
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 121464 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepYO323
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Vector typeYeast high copy 2 micron vector
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Selectable markersHIS3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCNB1mutEF2
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SpeciesS. cerevisiae (budding yeast)
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MutationChanged aspartic acid 66 to alanine
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GenBank IDNM_001179756
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Entrez GeneCNB1 (a.k.a. YKL190W, CRV1, YCN2)
- Promoter Endogenous genomic promoter region
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamH1 (not destroyed)
- 3′ cloning site Xho1 (not destroyed)
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTJK509 was a gift from Tami Kingsbury (Addgene plasmid # 121464 ; http://n2t.net/addgene:121464 ; RRID:Addgene_121464) -
For your References section:
Calcineurin Regulatory Subunit Calcium-Binding Domains Differentially Contribute to Calcineurin Signaling in Saccharomyces cerevisiae. Connolly S, Quasi-Woode D, Waldron L, Eberly C, Waters K, Muller EM, Kingsbury TJ. Genetics. 2018 Jul;209(3):801-813. doi: 10.1534/genetics.118.300911. Epub 2018 May 7. 10.1534/genetics.118.300911 PubMed 29735720