pTJK504
(Plasmid
#121459)
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PurposeCEN HIS3 CNB1mutEF2
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 121459 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRS313
- Backbone size w/o insert (bp) 4967
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Vector typeYeast CEN plasmid
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Selectable markersTRP1, HIS3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCNB1mutEF2
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Alt nameCalcineurin B
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SpeciesS. cerevisiae (budding yeast)
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MutationChanged aspartic acid 66 to alanine
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GenBank IDNM_001179756
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Entrez GeneCNB1 (a.k.a. YKL190W, CRV1, YCN2)
- Promoter Endogenous genomic promoter region
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer T3 promoter primer
- 3′ sequencing primer T7 promoter primer (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTJK504 was a gift from Tami Kingsbury (Addgene plasmid # 121459 ; http://n2t.net/addgene:121459 ; RRID:Addgene_121459) -
For your References section:
Calcineurin Regulatory Subunit Calcium-Binding Domains Differentially Contribute to Calcineurin Signaling in Saccharomyces cerevisiae. Connolly S, Quasi-Woode D, Waldron L, Eberly C, Waters K, Muller EM, Kingsbury TJ. Genetics. 2018 Jul;209(3):801-813. doi: 10.1534/genetics.118.300911. Epub 2018 May 7. 10.1534/genetics.118.300911 PubMed 29735720