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Addgene

pCDH-puro/Flag PD-L1 N192Q/N200Q
(Plasmid #121453)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 121453 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDH-puro
  • Backbone manufacturer
    System Bioscience
  • Backbone size w/o insert (bp) 7384
  • Total vector size (bp) 8384
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CD274
  • Alt name
    PD-L1 N192Q/N219Q
  • Species
    H. sapiens (human)
  • Mutation
    N192Q/N219Q
  • GenBank ID
  • Entrez Gene
    CD274 (a.k.a. B7-H, B7H1, PD-L1, PDCD1L1, PDCD1LG1, PDL1, hPD-L1)
  • Promoter CMV
  • Tag / Fusion Protein
    • Flag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer pCMVF
  • 3′ sequencing primer pCDHR
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDH-puro/Flag PD-L1 N192Q/N200Q was a gift from Mien-Chie Hung (Addgene plasmid # 121453 ; http://n2t.net/addgene:121453 ; RRID:Addgene_121453)

  • For your References section:

    Glycosylation and stabilization of programmed death ligand-1 suppresses T-cell activity. Li CW, Lim SO, Xia W, Lee HH, Chan LC, Kuo CW, Khoo KH, Chang SS, Cha JH, Kim T, Hsu JL, Wu Y, Hsu JM, Yamaguchi H, Ding Q, Wang Y, Yao J, Lee CC, Wu HJ, Sahin AA, Allison JP, Yu D, Hortobagyi GN, Hung MC. Nat Commun. 2016 Aug 30;7:12632. doi: 10.1038/ncomms12632. 10.1038/ncomms12632 PubMed 27572267
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