pCDH-puro/Flag PD-L1 N35Q
(Plasmid
#121449)
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Purposeexpression of human PD-L1 mutant in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 121449 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCDH-puro
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Backbone manufacturerSystem Bioscience
- Backbone size w/o insert (bp) 7384
- Total vector size (bp) 8384
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Vector typeMammalian Expression, Lentiviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCD274
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Alt namePD-L1
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SpeciesH. sapiens (human)
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Insert Size (bp)900
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MutationN35Q
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GenBank ID
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Entrez GeneCD274 (a.k.a. B7-H, B7H1, PD-L1, PDCD1L1, PDCD1LG1, PDL1, hPD-L1)
- Promoter CMV
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Tag
/ Fusion Protein
- Flag (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer pCMVF
- 3′ sequencing primer pCDHR (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCDH-puro/Flag PD-L1 N35Q was a gift from Mien-Chie Hung (Addgene plasmid # 121449 ; http://n2t.net/addgene:121449 ; RRID:Addgene_121449) -
For your References section:
Glycosylation and stabilization of programmed death ligand-1 suppresses T-cell activity. Li CW, Lim SO, Xia W, Lee HH, Chan LC, Kuo CW, Khoo KH, Chang SS, Cha JH, Kim T, Hsu JL, Wu Y, Hsu JM, Yamaguchi H, Ding Q, Wang Y, Yao J, Lee CC, Wu HJ, Sahin AA, Allison JP, Yu D, Hortobagyi GN, Hung MC. Nat Commun. 2016 Aug 30;7:12632. doi: 10.1038/ncomms12632. 10.1038/ncomms12632 PubMed 27572267