ACTB-PQR-RFPnols-PQR-loxP-Zeo-loxP
(Plasmid
#121448)
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PurposeTo make a stable human recombinant cell line; see right thumbnail map image for PQR annotations
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 121448 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCMV
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Vector typeMammalian Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRFPnols-PQR-ZeocinR
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Alt nameactin beta
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SpeciesH. sapiens (human)
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Entrez GeneACTB (a.k.a. BKRNS, BNS, BRWS1, CSMH, DDS1, PS1TP5BP1, THC8)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
ACTB-PQR-RFPnols-PQR-loxP-Zeo-loxP was a gift from Brian Chen (Addgene plasmid # 121448 ; http://n2t.net/addgene:121448 ; RRID:Addgene_121448) -
For your References section:
Generating stable cell lines with quantifiable protein production using CRISPR/Cas9-mediated knock-in. Lo CA, Greben AW, Chen BE. Biotechniques. 2017 Apr 1;62(4):165-174. doi: 10.2144/000114534. 10.2144/000114534 PubMed 28403807
Map uploaded by Addgene staff.
