RPL13A-PQR-RFPnols-PQR-loxP-Zeo-loxP
(Plasmid
#121447)
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PurposeTo make a stable human recombinant cell line; see right thumbnail map image for PQR annotations
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 121447 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCMV
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Vector typeMammalian Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameRFPnols-PQR-ZeocinR
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SpeciesH. sapiens (human)
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Entrez GeneRPL13A (a.k.a. L13A, TSTA1, uL13)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
RPL13A-PQR-RFPnols-PQR-loxP-Zeo-loxP was a gift from Brian Chen (Addgene plasmid # 121447) -
For your References section:
Generating stable cell lines with quantifiable protein production using CRISPR/Cas9-mediated knock-in. Lo CA, Greben AW, Chen BE. Biotechniques. 2017 Apr 1;62(4):165-174. doi: 10.2144/000114534. 10.2144/000114534 PubMed 28403807