Myc-gamma3/gamma2SIL
(Plasmid #121433)

• Purpose: GABAA receptor expression (chimeric rat gamma 3 subunit whose large intracellular loop has been replaced with the large intracellular loop of the rat gamma 2 short subunit) Myc-tag near N-terminus
• Depositing Lab: Angel de Blas
• Publication: GABAA receptor expression plasmids (unpublished)

Backbone

• Vector backbone: pCDNA3.1
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GABRG3/GABRG2 SIL
• Species: R. norvegicus (rat)
• Entrez Gene: Gabrg2
• Entrez Gene: Gabrg3
• Tag / Fusion Protein:
  • cMyc epitope EQKLISEEDL inserted between fourth and fifth amino acids of mature protein after signal peptide is cleaved (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (unknown if destroyed)
• 3′ cloning site: XhoI (unknown if destroyed)
• 5′ sequencing primer: unknown

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Rat gamma 3 and rat gamma 2 genes were originally cloned in Peter Seeburg’s lab in Heidelberg. Subcloning into different plasmids, tagging and making the chimeric constructs was done in Angel de Blas's lab.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry