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Addgene

pCu-MCP-2yeGFP(405)
(Plasmid #121419)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 121419 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS405
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CUP1p-MCP-2yeGFP
  • Alt name
    MS2 coat protein
  • Species
    Synthetic
  • Promoter CUP1 promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Xma1 (unknown if destroyed)
  • 3′ cloning site Sac2 (unknown if destroyed)
  • 5′ sequencing primer cattcgccattcaggctg
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCu-MCP-2yeGFP(405) was a gift from Daniel Klionsky (Addgene plasmid # 121419 ; http://n2t.net/addgene:121419 ; RRID:Addgene_121419)

  • For your References section:

    The Pat1-Lsm Complex Stabilizes ATG mRNA during Nitrogen Starvation-Induced Autophagy. Gatica D, Hu G, Liu X, Zhang N, Williamson PR, Klionsky DJ. Mol Cell. 2019 Jan 17;73(2):314-324.e4. doi: 10.1016/j.molcel.2018.11.002. Epub 2018 Dec 6. 10.1016/j.molcel.2018.11.002 PubMed 30527663
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